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Combined LC-MS (ESI in negative ion mode) chromatograms of ions at m/z 249, m/z 265, and m/z 281 corresponding to deprotonated TCEP, TCEP(O), and TCEP(S), respectively, for native lysozyme incubated with 10 mM TCEP (A) and lysozyme aggregates incubated with 0.5 mM TCEP (B).
