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. 2015 Feb 3;10(2):e0117099. doi: 10.1371/journal.pone.0117099

Fig 1. Construction of pET-GS-HNTX-IV expression vector.

Fig 1

A) Schematic diagram of RF-cloning strategy. F1: forward primer. R1: reverse primer. The portion complementary with the target gene is marked gray, and the portion complementary to the recipient vector is marked black. B) Map of pET-GS-HNTX-IV expression vector.