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. 2015 Feb 3;10(2):e0117099. doi: 10.1371/journal.pone.0117099

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© 2015 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — A) M: Protein molecular weight marker; 1–10: fractions extracted by TCA from 1% to 10%; P: precipitates. B) Relative purity (compared with lane 10) and relative yield (compared with lane 1) of rHNTX-IV under different TCA concentrations. The value was calculated by grayscale by Quantity One software. The data points (means ± S.E.) come from three independent experiments. C) Purification by RP-HPLC.