Fig. 1.

Schematic diagram of DNA constructs representing the expression cassettes inserted into the pGreen vector used as a backbone for the in vivo reconstitution of RNA1 and RNA2 of p35ToTV-Kra (upper panel). The detailed CaMV35S/cDNA/NOS junction site formed after recombination of pGreenMod1 and pGreenMod2 with ToTV-Kra cDNA1 and cDNA2, respectively, is indicated. The underlined nucleotide indicates the first transcribed nucleotide (lower panel). ProCo, protease cofactor; Hel, helicase; Pro, protease; RdRP, RNA-dependent RNA polymerase; ORF1, protein of unknown function; 3A, movement protein; Vp35, Vp26, Vp23, subunits of capsid and UTR, untranslated region