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. 2015 Feb 3;10(2):e0116594. doi: 10.1371/journal.pone.0116594

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Spf-J and WT were maintained on a regular chow diet (22% protein) and euthanized at 8 weeks. Blood was collected by retro-orbital bleeding with plasma separated and stored at −80°C until use. Tissues were snap frozen and stored at −80°C until use. A) mRNA level in spf-J (N = 4) and WT (N = 4) livers. B) Immunoblot of OTC protein. C) OTC enzyme activity in liver homogenates from spf-j (N = 6) and WT (N = 4). D) Plasma ammonia in spf-j (N = 7) and WT (N = 6). E) Plasma orotate in spf-j (N = 5) and WT (N = 6). Hatched bar indicates P < 0.05. WT – wild-type.