Skip to main content
. 2015 Jan 27;10:609–631. doi: 10.2147/IJN.S71198

Figure 2.

Figure 2

The 1H-NMR spectra of PLGA-PEG-POD and PLGA-PEG-HIV-Tat confirm the peptide coupling to PLGA-PEG-maleimide polymer.

Notes: 1H-NMR spectra in DMSO-d6 at 25°C of (A) PLGA-PEG-maleimide-POD copolymer and POD peptide. We used the specified amide/amine side chain region for peptide signal integration. (B) For PLGA-PEG-maleimide-HIV-Tat and PLGA-PEG-maleimide, we clearly see a reduction of the maleimide methine protons signal intensity and the appearance of Tyr aromatic protons from HIV-Tat peptide after conjugation.

Abbreviations: 1H-NMR, proton nuclear magnetic resonance; DMF, N,N-Dimethylformamide; DMSO, dimethyl sulfoxide-d6; HIV-Tat, human immunodeficiency virus transactivator; Mal, maleimide; PEG, polyethyleneglycol; PLGA, poly(lactic-co-glycolic acid); POD, peptide for ocular delivery; Tyr, tyrosine.