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. 2015 Feb 3;10(2):e0115593. doi: 10.1371/journal.pone.0115593

Figure 5. CD74 siRNA transfection inhibits MIF-induced eotaxin mRNA expression by cultured fibroblasts from WT mice.

Figure 5

CD74-specific siRNA was transfected into cultured mouse fibroblasts at a concentration of 1 μM. Forty-eight hours after transfection, the cells were stimulated with (A) IL-4 (10 ng ml-1), (B) IL-13 (10 ng ml-1) and MIF (100 ng ml-1) alone or in combination for 24 hours. The cells were harvested by the addition of lysis reagents. RNA was extracted from the cells, and the abundance of eotaxin mRNA was evaluated by reverse transcriptase-PCR. The data are from a representative experiment that was repeated three times and yielded similar results.