Fig 1. Experimental design to study the effects of isolated prostatic inflammation on voiding behavior.

For mice receiving NTF, NTF was injected sc, twice daily, beginning one day prior to transurethral instillation and continuing until the end of the experiment. Either saline or uropathogenic E.coli 1677 (2 x 10⁶ per ml), 200 µl per mouse, was instilled transurethral. (A) Histologic analysis and tissue culture was performed at the time points indicated for mice in the Naïve + NTF, Saline + NTF, E.coli + NTF and E.coli only groups. (B) Voiding behavior of mice in Saline + NTF and E.coli + NTF groups was determined by metabolic cage tests at PID 1, 4, 7 and 14.