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. 2015 Feb 2;10:10.3402/ljm.v10.26412. doi: 10.3402/ljm.v10.26412

Table 1.

Polymerase chain reaction (PCR) primer sequences for the detection of extended-spectrum β-lactamases genes in E. coli

Target gene Oligonucleotide primer sequences (5′ to 3′) Amplicon size (bp) Reference
bla TEM-1F CAGCGGTAAGATCCTTGAGA 643 11
bla TEM-1R ACTCCCCGTCGTGTAGATAA
bla SHV-1F GGCCGCGTAGGCATGATAGA 714 11
bla SHV-1R CCCGGCGATTTGCTGATTTC
bla OXA-1F AATGGCACCAGATTCAACTT 599 11
bla OXA-1R CTTGGCTTTTATGCTTGATG
bla CTX-M-1F GAAGGTCATCAAGAAGGTGCG 560 12
bla CTX-M-1R GCATTGCCACGCTTTTCATAG