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. 2014 Dec 1;190(11):1229–1242. doi: 10.1164/rccm.201405-0872OC

Figure 8.

Figure 8.

Protein phosphatase 2A (PP2A) activity is required for α1-antitrypsin (A1AT) to inhibit tumor necrosis factor (TNF)-α induced proteases and cytokines. A549 cells, which were stably transfected with lentivirus expressing control PP2AA or protein tyrosine phosphatase 1B (PTP1B) shRNA, were exposed to 2 μM A1AT for 1 hour before 10 ng/ml TNF-α stimulation (24 h). Quantitative polymerase chain reaction and multiplex analysis were performed for (A) matrix metalloproteinase (MMP)-1 and (B) MMP-9. (C) Multiplex analysis was performed for IL-8 and monocyte chemotactic protein (MCP) 1 and quantitative polymerase chain reaction was performed for IL-1β. Graphs represent mean ± SEM, where each measurement was performed three times (n = 6 per group). *P < 0.05 compared with A549 cells treated only with TNF-α. **P < 0.05 compared with A1AT+TNF-α–treated control shRNA A549 cells.