Table 3.

Plasmid and primers used in this study: entry clones and nongateway plasmids^a

Designation	Description	Primer 1	Primer 2	Vector	Description
TU#1114	mec-18p::atg	GGGGACAACTTTGTATAGAAAAGTTGAATTAATTCGTCTACTATCCACGTGTCGAT	GGGGACTGCTTTTTTGTACAAACTTGCCATGCTCACAACCTTCTTGGAAGGCG	PDONR-P4P1r	mec-18 promoter, to last gene, including ATG
TU#1107	cav-1	GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGAGATTGTGCAACGTGTGGAATG	GGGGACCACTTTGTACAAGAAAGCTGGGTGGACGCATGGAGCAGTAGTTTCTTG	PDONR221	cav-1 genomic DNA
TU#1108	mfb-1c	GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGCCATTCATTGGACGTGATTGG	GGGGACCACTTTGTACAAGAAAGCTGGGTGTTACAAATAAATCAACATATCGACAAATTGTCTGGG	PDONR221	mfb-1 cDNA
TU#1102	utr	GGGGACAGCTTTCTTGTACAAAGTGGTTTAGCATTCGTAGAATTCCAACTGAGCG	GGGGCCTTTGTTAATAAAGTTGGGAAACAGTTATGTTTGGTATATTGGGAATG	pDONR-P2rP3	unc-54 3′UTR from pPD95.75
TU#1103	gfp::utr	GGGGACAGCTTTCTTGTACAAAGTGGTTTGGCCAAAGGACCCAAAGGTATG	GGGGCCTTTGTTAATAAAGTTGGGAAACAGTTATGTTTGGTATATTGGGAATG	pDONR-P2rP3	gfp + unc-54 3′UTR from pPD95.75
pCW2.1	ceh-22p::gfp				ceh-22p::gfp (Okkema and Fire, 1994)
pCFJ90	myo-2p::mCherry				Addgene (Frokjaer-Jensen et al., 2008)
TU#929	mec-3p::mrfp			pPD95.77_HcRed1	1.9kb Pst-1/BamHI mec-3 promoter sequence inserted into modified pPD95.77 carrying mTagRFP (generated by Irini Topalidou, pers. comm.)

^aThe primer sequences used to PCR the inserted sequences were provided.