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Western blotting of A1M before and after MPO-induced cleavage. The samples, 0.1 μg of full-length A1M, MPO and a mixture of A1M/MPO, 0.1 μg of each, incubated for 4 h, were separated on a 12% SDS-PAGE prior to transfer to a PVDF-membrane and blotting. (A) Staining with Coomassie. (B) Immunoblot with anti-LIPR antibodies.
