Figure 6.

Cleavage of A1M in neutrophil culture medium. SDS-PAGE on 12% gels in the presence of mercaptoethanol followed by Western blotting with anti-A1M antibodies. The left lanes show A1M (0.1 mg/ml) incubated in fresh medium, the middle lanes show A1M incubated with neutrophil culture medium (0.1 mg/ml), and the right lanes show A1M (0.1 mg/ml) incubated with culture medium together with neutrophils activated by ionomycin. Culture conditions are described in Materials and Methods. Left panel, incubation for 3 h, and right panel shows the same procedure but with 24 h incubation.