. 2014 Jan 3;9(4):526–537. doi: 10.1002/biot.201300305

Table 2.

Overview of the results from the fed batch cultivations

	P. pastoris 3D6scFv-Fc	CHO 3D6scFv-Fc	P. pastoris HSA	CHO HSA^e)
Duration (h)	145	234	149	379
Maximal cell dry mass concentration CDM_max (g L^–1)^a)	85.3 ± 1.0	0.604 ± 0.027	94.1 ± 4.2	0.311 ± 0.009
Maximal specific growth rate μ_max (h^–1)^a)	0.147 ± 0.008	0.028 ± 0.002	0.148 ± 0.003	0.023 ± 0.002
Gene copy number	10 ± 1^c)	18 ± 2^d)	11 ± 2^c)	130 ± 16^d)
Product concentration (supernatant) (mg L^–1)^a)	12.3 ± 0.1	159.4 ± 0.7	551.0 ± 36.4	129.4
Product concentration (broth) (mg L^–1)^a)	8.8 ± 0.1	158.9 ± 0.6	380.2 ± 32.7	129.3
Mean specific secretion rate q_{P, mean} (feed phase) (mg g^–1 h^–1)^b)	0.0018	1.82	0.071	1.86
Maximal specific secretion rate q_{P, max} (feed phase) (mg g^–1 h^–1)^b)	0.0040	2.36	0.113	2.77
Space-time yield (process end) STY_end (mg L^–1 h^–1)^b)	0.067	0.642	2.856	0.312
Maximal space-time yield STY_max (feed phase) (mg L^–1 h^–1)^b)	0.088	0.642	2.866	0.427

^a)

Mean ± standard deviation (n = 2), two independent fed batch cultivations.

^b)

Calculated with smoothed product concentrations, two independent fed batch cultivations.

^c)

Mean ± standard deviation (n = 4), data from strain selection.

^d)

Mean ± standard deviation (n = 2), data from cell line selection.

^e)

Data of one cultivation after 163 h.