Fig. 8.

Effects of EFV on the hepatocellular NADH/NAD⁺ ratio and on mitochondrial protein acetylation. (A) Hepatocytes were exposed to EFV (50 µM) for 3 h, and NADH/NAD⁺ levels were determined. *P<0.05 versus solvent control. (B) Abundance of acetylated lysine (Ac-Lys) of CypD in mitochondria isolated from solvent controls or EFV (50 µM)-treated hepatocytes (4.5 h exposure). The Ac-Lys antibody was used to immunoprecipitate mitochondrial proteins, and the immunoblot analysis was performed with anti-CypD antibody. The CypD immunoblot analysis shows equal loading of samples.