Table 1.
PCR primer sequences.
| Gene | Forward 5'-3’ | Reverse 5'-3’ |
| Cytokines | ||
| IL-1b | GAGATTGAGCTGTCTGCTCA | AAGGAGAACCAAGCAACGAC |
| IL-6 | GATGCTACCAAACTGGATATAATC | GGTCCTTAGCCACTCCTTCTGTG |
| IL-18 | ACTGTACAACCGCAGTAATACGG | AGTGAACATTACAGATTTATCCC |
| TNFa | TGGGAGTAGACAAGGTACAACCC | CATCTTCTCAAAATTCGAGTGACAA |
| ENA78 (CXCL5) | CTTCCTCAGTCATAGCCGCAAC | ATCCGTGGGTGGAGAGAATCAG |
| IP-10 (CXCL10) | TTTCTGCCTCATCCTGCTGG | GGAGCCCTTTTAGACCTTTTTTGG |
| MIP-1a (CCL3) | GCCCTTGCTGTTCTTCTCTGT | GGCATTCAGTTCCAGGTCAGT |
| MIP-1b (CCL4) | ACACCATGAAGCTCTGCGT | CGCTGGAGCTGCTCAGTTC |
| MIP-2a (CXCL2) | CAAAGGCAAGGCTAACTG | TGTTCTACTCTCCTCGGT |
| MIP-3a (CCL-20) | GCAGAAGCAGCAAGCAACTACG | GAGGTTCACAGCCCTTTTCACC |
| MCP-1 (CCL2) | CTCACCTGCTGCTACTCATTC | GCTTGAGGTGGTTGTGGAAAA |
| MCP-3 (CCL7) | TGTGCCTGAACAGAAACCAACC | AAAAATGGGGAAAGGGGGAG |
| KC (GRO) | GTCCTTTGAACGTCTCTGTC | GCTGGCTTCTGACAACACTA |
| RANTES (CCL5) | CAAGGAGTATTTCTACACCAGCAGC | ATGCCGATTTTCCCAGGACC |
| MEC (CCL-28) | GCTGTGTGTGTGGCTTTTCAAAC | TTCTGTCCTTCCTGCTGGGTTG |
| TLR- signaling | ||
| MyD88 | ACCCCACTCGCAGTTTGTTGGATG | TGGTGATGCCTCCCAGTTCCTTTG |
| TIRAP | CAATCTACCTGGAATCGGCTGTC | GCATCTTCTTGGGCTTCTTCAAC |
| IRAK | TGCCGCTTCTACAAAGTGATGGACG | AATGGGTCTGGGAGCCTGGAAAAG |
| Caspase-1 | ATTACTGCTATGGACAAGGCACGG | CCCTCGGAGAAAGATGTTGAAACTC |
| TRAF-6 | AATGGAAGCACAGCAGTGTAACGGG | AAGGCAAGCAGTTCTGGTTTGGCG |
| IKKb | TGGAGCCTGGGAAATGAAAGAACG | TCTAAGAGCCGATGCGATGTCACTC |
| IKKa | AGCCATTCCTCACTTACCAGTCCG | CAGCAGTCTCTTCCACAGAAAACCC |
| Others | ||
| CD45 | CTTCAGAGCCTCGTACCAGC | TGTGTCCAGAAGGGCAAATC |
| b-actin | GCTTCTTTGCAGCTCCTTCGTTG | TTCTCCATGTCGTCCCAGTTGG |
| Vimentin | CTGTGTCCTCGTCCTCCTACC | GCAGTTCTACCTTCTCGTTGG |
| b-defensin3 | GCATTTGAGGAAAGGAACTCCACAAC | GTCTCCACCTGCAGCTTTTAGCAA |
| Angiogenin4 | CTCTGGCTCAGAATGTAAGGTACGA | GAAATCTTTAAAGGCTCGGTACCC |
| SAA-1 | GTAATTGGGGTCTTTGCC | TTCTGCTCCCTGCTCCTG |