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. 2015 Feb 7;21(5):1498–1509. doi: 10.3748/wjg.v21.i5.1498

Figure 2.

Figure 2

Dose-dependent increases in hepatitis B virus DNA and RNA in stable hepatitis B virus-expressing cell lines after treatment with vincristine. A: Dose-dependent increase in HBV DNA in HepG2-HBV1.1 and HepG2.2.15 cells treated with vincristine based on real-time fluorescent quantitative PCR analysis; B: Dose-dependent increase in HBV DNA in HepG2-HBV1.1 and HepG2.2.15 cells treated with vincristine based on Southern blot analysis; C: Vincristine increases HBV DNA in the supernatants of HepG2-HBV1.1 cells and HepG2.2.15 cells based on real-time fluorescent quantitative PCR analysis; D: Vincristine increases HBV DNA in the supernatants of HepG2-HBV1.1 cells and HepG2.2.15 cells based on Southern blot analysis; E: Vincristine up-regulates HBV pgRNA based on real-time fluorescent quantitative PCR analysis. HepG2-HBV1.1 (6 × 105/well) and HepG2.2.15 (4 × 105/well) cells were treated with 0.1 μmol/L vincristine for 24 h followed by 48 h of incubation in drug-free culture medium. HBV intermediates or total RNA were extracted 72 h after drug treatment. Values represent the mean ± SD of 3 independent experiments, and a representative experiment is shown. aP < 0.05, bP < 0.01 vs control group (no vincristine). rc: Relaxed circular DNA; ds: Double-stranded DNA; ss: Single-stranded DNA. M: DNA ladder; pgRNA: Pregenome RNA.