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. 2015 Feb 7;21(5):1498–1509. doi: 10.3748/wjg.v21.i5.1498

Figure 3.

Figure 3

Vincristine promotes hepatitis B virus protein expression in HepG2-HBV1.1 and HepG2.2.15 cells. A: Vincristine up-regulates HBeAg expression. HBeAg in the supernatant was analyzed using an ELISA assay; B: Vincristine increases cellular HBcAg expression based on Western blot analysis; C: The effect of vincristine on intracellular HBsAg expression was analyzed using an ELISA assay; D: The effect of vincristine on HBsAg expression in the supernatant was analyzed using an ELISA assay. HepG2-HBV1.1 (6 × 105/well) and HepG2.2.15 (4 × 105/well) cells were treated with vincristine at a concentration of 0.1 μmol/L for 24 h followed by 48 h of incubation in drug-free culture medium. Values represent the mean ± SD of 3 independent experiments. aP < 0.05, bP < 0.01 vs control group (no vincristine). HBeAg: Hepatitis B e Antigen; HBcAg: Hepatitis B c Antigen; HBsAg: Hepatitis B surface antigen.