Fig. 1.

Gel electrophoresis, agarose 2% (w/v) for codon 12. PCR product (left, band at 135 bp) and restriction fragments (right) were migrated for each sample; NTC – no template control; PC – positive control (mutant heterozygous, bands at 135, 106 and 29 bp); NC – negative control (wild type, bands at 106 and 29 bp); WT – wild type sample, M – mutant sample; L – DNA molecular weight markers (GeneRuler 50 pb DNA Ladder, Fermentas)