Table 1. Specific activities of bacterial peroxidase using various substrates.
Origin | Biocatalyst | Substrate | Assay condition | Specific activity (U/mg protein) | Ref. | |
---|---|---|---|---|---|---|
pH | Temp. (°C) | |||||
Thermobifida fusca | DyP | Reactive Blue19 | 3.5 | 25 | 4.28 | 22 |
Reactive Black5 | 3.5 | 25 | 0.06 | |||
Guaiacol | 3.5 | 25 | 0.03 | |||
2,6-dimethoxyphenol | 3.5 | 25 | 0.17 | |||
VA | 3.5 | 25 | 0.01 | |||
Anabaena species | DyP | Reactive Blue19 | 4.8 | 35 | 401 | 30 |
Reactive Black5 | 4.4 | 35 | 21 | |||
Rhodococcus jostii RHA1 | DyP | ABTS | 5.0 | 30 | 1.54a | 12 |
ABTS | 5.0 | 30 | 13.09b | |||
GGE | 5.5 | 30 | 0.017b | |||
Pseudomonas putida MET94 | DyP | ABTS | 5 | 10–30 | 40 | 26 |
VA | 5 | 10–30 | 6.2 × 10−6 | |||
Bacillus subtilis | DyP | ABTS | 4 | 20–30 | 15 | |
Acinetobacter calcoaceticus | LiP | VA | 1.0 | 50 | 0.043 | 23 |
Bacillus subtilis | DyP | ABTS | 3.0 | 50 | 66.80 | This study |
VA | 3.0 | 50 | 0.13 | |||
VGE | 3.0 | 50 | 0.086 | |||
Reactive Blue19 | 3.0 | 30 | 11.55 | |||
Reactive Black5 | 3.0 | 30 | 17.65 |
One unit (U) corresponds to 1 μmole of product per minute.
aAssayed without MnCl2;
bAssayed with MnCl2.