Fig. 5.

HDAC genes HDA1 and RPD3 are involved in repression of FLO1 gene transcription. (A) Northern blot analysis of FLO1 mRNA in wt and strains deleted for the HDAC genes indicated. The blot was re-probed with ACT1 as a loading control. Two lanes between the rpd3 hos1 hos2 and cyc8 samples were left empty to prevent ‘bleed’ on the blot from the high signal expected in the cyc8 sample. (B) Analysis of flocculation phenotype in wt and strains deleted for the HDAC genes indicated. Flocculation was assayed by photographing yeast cells in liquid culture five minutes after cessation of agitation.