Fig. 6.

Histone occupancy and histone acetylation are altered in an rpd3 hda1 double mutant. (A) Histone occupancy is reduced in the absence of Rpd3p and Hda1p. ChIP analysis using antibodies against histone H3. Graph plotting histone H3 occupancy at the FLO1 promoter and upstream region in wt, rpd3, hda1 and rpd3 hda1 deletion strains. Histone H3 occupancies were normalised to the ORF-free region, INT-V. The results represent means from three to four independent experiments, with bars depicting SEM. The wt data shown were to aid comparison, and are the same data as shown in Fig. 4A. (B and C) Histone H4 and H3 acetylation levels increase across the FLO1 promoter and upstream region in the absence of Hda1p and Rpd3p. The level of acetylated histones H4 (B) and H3 (C) over the FLO1 promoter and upstream region in wt, rpd3, hda1 and rpd3 hda1 deletion strains was determined by ChIP as described in Fig. 4. H4ac and H3K9ac occupancy was normalised to histone H3 levels. The results represent the mean from three to four independent experiments, with bars depicting SEM. The wt data were to aid comparison, and are the same data as shown in Figs. 4C and D. (A–C) The amplicon positions are as described in Fig. 2A.