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. Author manuscript; available in PMC: 2015 Feb 4.
Published in final edited form as: Radiat Res. 2011 Sep 30;177(2):176–186. doi: 10.1667/rr2580.1

FIG. 3.

FIG. 3

Radiation-induced γ-H2AX focus formation. Panel A: Cells were harvested 24 h after irradiation, stained for γ-H2AX detection (green), counterstained with DAPI (blue), and scored for nuclei with >4 foci. One hundred nuclei were counted per sample (31). WT1 and 5 and AT3 and 4 cells were used as controls. Panel B: Normalization of γ-H2AX focus formation 24 h postirradiation to nonirradiated (spontaneous) focus formation in panel A. Error bars represent means ± SD. N = 3. Inset: representative data for RS50.