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. 2015 Feb 4;5:8234. doi: 10.1038/srep08234

Figure 4. The downregulation of IL-17A, IL-17F and the RORγt expressions in the Jagged-1-treated CD4+ T cells.

Figure 4

The isolated CD4+ T cells were treated with Jagged-1 for 72 h. (A–C) The relative quantification of Il-17a, Il-17f and Rorγt mRNA expressions was detected using 2−ΔΔCt method by qPCR. (D–F) The levels of IL-17A, IL-17F and RORγt proteins were determined by Western Blot. (G and H) The levels of IL-17A and IL-17F secreted by the treated CD4+ T cells were measured by ELISA. The results are representative of the three independent experiments. *p < 0.05, **p < 0.01.