Figure 3.

A blocker of Src family kinases PP2 increased serotonin immunoreactivity caudal to the lesion epicenter after spinal cord injury.

(A) Immunoreactive fibers for serotonin (5-hydroxytryptamine) (white arrowheads) were observed caudal (approximately 2 mm) to the lesion epicenter in PP2-treated rats but very few in PP3-treated and DMSO-treated rats. Scale bar: 30 μm. (B) Statistical analysis shows an increase in 5-hydroxytryptamine fiber density caudal to the lesion epicenter in PP2-treated rats (**P < 0.01 for PP3-treated rats and ***P < 0.001 for DMSO-treated rats) (n = 3). One-way analysis of variance followed by Bonferroni post hoc test was used to determine the significant differences among samples studied (F_(2,6) = 34.80, P = 0.0005). Error bars show the standard error of the mean (SEM). PP2: 4-Amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyramidine; PP3: 4-amino-7-phenylpyrazolo[3,4-d]pyramidine (the inactive analog of PP2); DMSO: dimethyl sulfoxide.