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. 2014 Dec 15;9(24):2205–2210. doi: 10.4103/1673-5374.147954

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Copyright: © Neural Regeneration Research

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Western blot assay for AQP4 and GFAP protein levels at 12 (A), 24 (B), 48 (C) and 72 hours (D) after injury.

The graph showed significant up-regulation of AQP4 and GFAP expression in the SCI group at 12, 24, 48 and 72 hours after SCI. Treatment of melatonin (100 mg/kg) could markedly decrease AQP4 and GFAP levels in the spinal cord at 24, 48 and 72 hours after injury. *P < 0.05, vs. sham surgery (sham) group; #P < 0.05, vs. SCI group. Data are expressed as the mean ± SD (n = 5, one-way analysis of variance and the least significant difference test). SCI: Spinal cord injury; AQP4: aquaporin-4; GFAP: glial fibrillary acidic protein.