Gene expression analysis. Relative mRNA expression of (A) COL2A1, (B) COL1A1, (C) aggrecan, (D) SOX9, (E) MMP1, (F) MMP13, (G) TIMP1, and (H) TIMP2 cultured at 32°C, 37°C, and 41°C for 2 days are shown (n = 3). Values represent the means and SD. *P < 0.05. **P < 0.01.