. 2014 Dec 24;7(1):391–409. doi: 10.1093/gbe/evu286

Table 2.

Primers Used for this Study

Forward	Primer	Reverse	Primer	Start	End	Size (bp)	Overlap
1F	ATGAACAAATATCTTACACG	1R	ATAARTGCTGRAATAAAAT	1	699	698	162
2F	ACAACATTTTTTGATCCT	2R	GCTAAACCCAAGAAATG	667	1,290	623	32
3F	ACAGGTTATAGTTATAATGA	3R	GTCTGCTGGCACTTAGTTAG	1,223	1,860	637	67
4F	CTGGTCGAAGATGCGTAGTA	4R	TGTGCTAACACTGGGTTAGA	1,743	2,500	757	117
5F	TATGCGCTACATTCTCCTAT	5R	CACACTTCATAGCTAATCAT	2405	3,128	723	95
ssRNA-F1	CTGCGTTTAATACGTACTTGGC	6R	YACTGCATCTAAACCTATCA	2,680	3,591	911	448
7F	ATTCTAGGAATGGGCTGC	7R	GACATTTGTCCCCAAGGTAA	3,509	4,126	617	82
8F	ATATTTTAAGAGACGTTAAT	8R	CTCTACTGGATTAGCCCCTA	3,964	4,726	762	162
9F^a	ATCCTTTAGTAACTCCTG	msh2806R	TAACTCAGCTTGAGAGTATGC	4,501	5,088	587	225
9F^a	ATCCTTTAGTAACTCCTG	msh3101R	GATATCACATAAGATAATTCCG	4,527	5,354	827	561
10F	YTRCTTCAAATGGGGTTTCC	mutS-3458R	TSGAGCAAAAGCCACTCC	5,268	5,731	463	86
10F	YTRCTTCAAATGGGGTTTCC	mutS-6088R^a	TGTGATAGGGTTGAGAAG	5,268	5,900	632	463
10F	YTRCTTCAAATGGGGTTTCC	10R	AGAATTGTAACACTCGGG	5,268	5,939	671	632
mutS-F5	ATTTAATTAAGAATCTCCAACTTCC	mutS-6979^a	TATTAATGGGTGTCGGAG	5,932	6,937	1,005	7
mutS-6818F^a	CTAAGCTATTTTTWCCCC	mutS-R2	TCTAAAGACTCATTAAGATAAACCC	6,918	7,875	957	19
13R	CTGTTTCCAAGCCTACTT	13F	CTATTTTAGGYTGGAAGAGA	7,861	8,623	762	14
14R	TTTCCTCTTGAGACAGTA	14F	ACTGGTGTAGTAAGACTA	8,516	9,219	703	107
octo2-H	CGATAAGAACTCTCCGACAATA	15F	CAACTGAATGGCCGCGGTAA	9,134	9,601	467	85
octo1-L	AGACCCTATCGAGCTTTACTGG	nd2-R1	GTTCAAGCTCTCCTGTGGAGCC	9,343	10,394	1051	258
nd2-1418R	ACATCGGGAGCCCACATA	16S-647F	ACACAGCTCGGTTTCTATCTACAA	9,772	10,552	780	622
16R	GCACGATAGATAATAGCGCA	16F	TGGTGACACAGCTCGGTT	9,791	10,590	799	761
17R	ATATTTGTTATTACTAAAGG	17F	ATTRTTATTTAAAGTATCTG	10,527	11,153	626	63
18R	TCCCAACCRATAAATARTTG	18F	GTTTTTAACTAARTGGTATR	11,043	11,709	666	110
19R	GCATGAATRATTGAGCCTGC	19F	ATTCTACAAGTTATATGAGA	11,605	12,323	718	104
20R	TATCATTAATGCATAATTAA	20F	AGTTTATATCAYYTACTAAC	12,299	13,051	752	24
21R	AACATTAAACTGAGCCGACT	21F	TGTCTCTTATCGTACTATAG	13,005	13,653	648	46
22R	TTTTATTATTAGTTAACCTTCATC	nad4-F3	TTTTATTATTAGTTAACCTTCATC	13,514	14,179	665	139
22R	GTACTAGTWGAAAAAGCAGC	nd4-13343F^a	AATAGGTTGGTTTGAGGG	13,514	14,300	786	665
co3bam567F	GCTGCTAGTTGGTATTGGCAT	23F	ATGGTRTTTACTTTAGCTAA	14,264	14,787	523	36
23R	GCTGCTAGTTGGTATTGGCA	23F	ATGGTRTTTACTTTAGCTAA	14,274	14,835	561	513
24R	TATCACCCTTATCATYTAGT	24F	CTAAGARCCCCACCARTAAA	14,772	15,508	736	63
25R	TCWACAGCTAAYAAGGGAAC	25F	TGAAAATATARTACTGAGCC	15,468	16,063	595	40
siro-cox2-F1	AGGCCCACTCTGTATATTTC	atp6-R2	ATGTAGATTTAGAGTATCATTAATRTA	15,588	16,291	703	475
26R	CATTAGSTATTAAAATGGAT	26F	GTAAATACRTAGGGAAATAG	15,524	16,597	1,073	767
cox2-16530F^a	CCCCTAAAGATCACCACA	nd42599F	GCCATTATGGTTAACTATTAC	16,582	17,397	815	15
27F	GAGTGATTAGCGCCACATAA	27R	GGAGCCTATATCCTTGRGAT	16,681	17,468	787	716
REVNRnd6^a	ATCGTTAGCGGGACATTATCAATT	coII-8068F	CCATAACAGGACTAGCAGCATC	17,207	17,995	788	261
nd6-F	TCCTTAGGAATAGTTGGAGCTAG	nd3-2126R	CACATTCATAGACCGACACTT	17,935	18,600	665	60
siro-nad6-R1	ATTGCCCCTATGTTAGTTCTAG	28R	CCAATCATTACTGGCATTAC	18,304	233	982	296
nd6-F REV	CTAGCTCCAACTATTCCTAAGGA	New NCR2R	ATGATCATCTCCTAACATACTACC	18,774	162	585	162
9F^b	ATCCTTTAGTAACTCCTG	COII-8068F	CCATAACAGGACTAGCAGCATC	4,531	5,123	593	—
msh2806R^b	TAACTCAGCTTGAGAGTATGC	RevNrND6	ATCGTTAGCGGGACATTATCAATT	17,209	18,037	829	—

Note.—Unless otherwise noted, sequence numbers are based on mt genomes with konojoi gene arrangement, starting with cox1.

All primers are from previous research (Brugler and France 2008; Uda et al. 2011; Park et al. 2012, Figueroa and Baco 2014).

^aPrimer pairs used for mt genomes with konojoi arrangement only.

^bPrimer pairs used for mt genomes with japonicum arrangement only.