Table 3.
Short characteristic of the proteins differentially expressed in HV, CKD and CVD groups
| Protein | Accession | P value (ANOVA) | Molecular function (GO) | Biological process (GO) | Relative abundance ± SD | ||||
|---|---|---|---|---|---|---|---|---|---|
| HV | CKD 1-2 | CKD 3-4 | CKD 5 | CVD | |||||
| α-1-microglobulin I | AMBP_Human | < 0.0001 | peptidase activity; protein binding; serine-type endopeptidaseinhibitor activity | immune system process; proteolysis; regulation of catalytic activity; metabolic process | 0.104 ± 0.03 | 0.129 ± 0.03 | 0.249 ± 0.08 | 0.273 ± 0.06 | 0.206 ± 0.04 |
| α-1-microglobulin II | AMBP_ Human | < 0.0001 | 0.091 ± 0.02 | 0.157 ± 0.04 | 0.289 ± 0.07 | 0.371 ± 0.05 | 0.138 ± 0.03 | ||
| Apolipoprotein A-IV | APOA4_ Human | < 0.0001 | enzyme regulatoractivity; protein binding; lipid binding transporter activity | cell communication; regulation of catalytic activity; response to stimulus; transport; blood circulation; lipid metabolic process | 1.033 ± 0.16 | 1.747 ± 0.17 | 2.260 ± 0.24 | 2.378 ± 0.25 | 0.574 ± 0.15 |
| Apolipoprotein A-I | APOA1_ Human | < 0.0001 | enzyme regulator activity; protein binding; lipid binding; transporter activity | cell communication; regulation of catalytic activity; response to stimulus; transport; blood circulation; lipid metabolic process | 4.25 ± 0.17 | 3.244 ± 0.13 | 3.116 ± 0.67 | 3.581 ± 0.68 | 2.00 ± 0.17 |
| Apolipoprotein B-100 | APOB_ Human | < 0.0001 | protein binding; lipid binding; transporter activity | cell communication; regulation of catalytic activity; response to stimulus; transport; blood circulation; lipid metabolic process | 0.579 ± 0.33 | 1.080 ± 0.10 | 1.907 ± 0.18 | 1.649 ± 0.19 | 1.207 ± 0.19 |
| α-2-macroglobulin | A2MG_ Human | < 0.0001 | peptidase activity; cytokine activity; serine-type endopeptidase inhibitor activity | complement activation; proteolysis; cell communication; response to stimulus; regulation of catalytic activity; lipid metabolic process; platelet activation | 1.163 ± 0.10 | 1.202 ± 0.13 | 1.995 ± 0.13 | 1.961 ± 0.14 | 1.104 ± 0.16 |
| Transthyretin | TTHY_ Human | < 0.001 | protein binding; transmembrane transporter activity | cell communication; transport; metabolic process; regulation of catalytic activity; response to stimulus | 2.116 ± 0.50 | 2.487 ± 0.81 | 2.617 ± 0.55 | 3.791 ± 0.86 | 2.476 ± 0.85 |
| Inter-α-trypsin inhibitor heavy chain H4 | ITIH4_ Human | < 0.0001 | peptidase activity; protein binding; serine-type endopeptidase 7inhibitor activity | proteolysis; immune system process; regulation of catalytic activity; response to stimulus | 1.492 ± 0.18 | 1.735 ± 0.07 | 1.861 ± 0.08 | 1.913 ± 0.06 | 2.396 ± 0.11 |
| Complement factor B | CFAB_ Human | < 0.0001 | peptidase activity; cytokine activity; serine-type endopeptidase inhibitor activity | cell communication; blood coagulation; immune system process; metabolic process; regulation of catalytic activity; response to stimulus; transport; proteolysis | 0.363 ± 0.07 | 0.520 ± 0.06 | 0.593 ± 0.06 | 0.510 ± 0.05 | 0.371 ± 0.06 |
| Haptoglobin | HPT_ Human | < 0.0001 | serine-type peptidase activity; calcium ion binding; calmodulin binding; calcium-dependent phospholipid binding | gamete generation; immune system process; proteolysis; cell communication; blood circulation; response to stress; blood coagulation; metabolic process | 1.073 ± 0.57 | 3.756 ± 0.59 | 3.970 ± 0.25 | 2.361 ± 0.65 | 2.060 ± 0.62 |
| Fibrinogen γ | FIBG_ Human | < 0.0001 | receptor binding | cell communication; cell-matrix adhesion; cell-cell adhesion; blood coagulation; | 1.677 ± 0.63 | 6.039 ± 0.79 | 6.626 ± 1.13 | 4.714 ± 0.79 | 3.304 ± 0.72 |
| Complement factor C4A | CO4A_Human | < 0.0001 | peptidase activity; cytokine activity; serine-type endopeptidase inhibitor activity | immune system process; proteolysis; cell communication; response to stimulus; regulation of catalytic activity | 5.98 ± 0.81 | 36.33 ± 4.61 | 53.98 ± 3.89 | 36.07 ± 0.63 | |
| Coiled-coil domain-containing protein 167 | CC167_HUMAN | < 0.0001 | No hit | No hit | 2.72 ± 0.52 | 2.16 ± 0.07 | 6.2 ± 0.98 | 2.25 ± 0.39 | |
| Putative uncharacterized protein LOC100129027 | YK026_HUMAN | < 0.0001 | No hit | No hit | 0.91 ± 0.09 | 1.46 ± 0.60 | 3.38 ± 0.36 | 1.48 ± 0.13 | |
The spots corresponding to these proteins showed at least a 1.6-fold increase or reduction of relative abundance. Molecular functions and biological processes for all proteins were defined using GO annotations. Relative accumulation of proteins was calculated on the basis of the average protein abundance for all samples of each group. SD means the standard deviation of protein abundance of one certain spot.