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. Author manuscript; available in PMC: 2015 Feb 4.
Published in final edited form as: Cell Cycle. 2007 Sep 4;6(23):2859–2868. doi: 10.4161/cc.6.23.5018

Figure 1.

Figure 1

TLR8 expression and localization in cultured cortical neurons. (A) TLR8 (red) is expressed intracellularly in cultured neurons and is enriched in their growth cones (arrowheads). (B) TLR8 (green)- positive punctuate staining is distributed along axons (neurofilament 200 KD, red) and is concentrated in growth cones (arrowheads). Cortical neurons are cultured from embryonic day 17 (E17) mice, fixed at day-in-vitro (DIV) 3 with methanol at -20°C for 10 minutes, followed by staining with anti-TLR8 polyclonal antibody alone (A), or together with anti-neurofilament 200 KD monoclonal antibody (B).

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