Figure 2.
Neurons do not express TLR7. (A-B) Immunostaining with anti-TLR7 polyclonal antibody on sagittal sections of E18 brain shows that TLR7 (red) is expressed only in the choroid plexus (arrowheads) and ependymal cells in the lateral ventricle (A) and fourth ventricle (B). (C) In the adult mouse spinal cord, TLR7 (red) is specifically expressed in ependymal cells (arrowheads) of the central canal. (D) Immunostaining of cortical neuron cultures from E18 mouse confirms that TLR7 is absent in neurons (MAP2, green; cell nuclei DAPI-stained, blue) but rather expressed in a few non-neuronal cells (red).