Skip to main content
. 2015 Jan 29;6:6003. doi: 10.1038/ncomms7003

Figure 4. QC expression of ROW1 reduced the amount of WOX5 transcripts dramatically with a resultant loss of DSC.

Figure 4

(a) GFP signals (green) were observed in QC and RFP signals (red) from mCherry-ROW1 expression were observed in the PM from plants carrying both WOX5::GFP and ROW1::mCherry-ROW1 constructs (upper panel), whereas only the RFP signals from mCherry-ROW1 expression were detected from the plants carrying both WOX5::GFP and WOX5::mCherry-ROW1 constructs (lower panel). (b) Root tips of WOX5::GFP plants showed normal QC (blue arrow) and DSC (red arrow, no starch granule) development, whereas starch granules appeared after staining with Lugol’s solution in the root tips of WOX5::GFP/WOX5::mCherry-ROW1 plants. (c) EdU assay showing normal mitotic activity in wild-type (col) root tip, in root tips of wild-type plants that express the WOX5::GFP construct (WOX5::GFP), whereas no such mitotic activity was observed in the same cell layer in plants expressed the WOX5::mCherry-ROW1 construct, similar to that of wox5-1. Yellow box, QC position; The white box below, DSC. (d) Three-dimensional reconstruction showing disrupted columella cell structure in 5-day-old root tips of both wox5-1 and WOX5::GFP/WOX5::mCherry-ROW1. (e) A working model depicts the involvement of ROW1 during development of the Arabidopsis root stem cell niche. Scale bars, 20 μm in this figure.