Table 2.
Comparison of Fc variants selected during MS1 and MS2.
| Fc variants | Mutations | Ratio/Fc-WT | Variant origin |
|---|---|---|---|
| C6A-69 | T307A/N315D/A330V/E382V/N389T/N434Y | 28.4 | MS2 |
| C6A-78 | T256N/A378V/S383N/N434Y | 27.8 | MS2 |
| C6A-78A | T256N/A378V/N434Y | 27.8 | MS2 + mutagenesis |
| T5A-74 | N315D/A330V/N361D/A378V/N434Y | 27.6 | MS2 |
| C6A-74 | V259I/N315D/N434Y | 27.2 | MS2 |
| C6A-60 | P230S/N315D/M428L/N434Y | 26.8 | MS2 |
| C6A-66 | E294del/T307P/N434Y | 24.6 | MS2 |
| T6A-21 | N315D/A378V/N434Y | 10.0 | MS2 |
| T4A-04 | N315D/A330V/E382V/N434Y | 9.6 | MS2 |
| S5A-41 | P230T/V303A/K322R/N389T/ | 9.0 | MS1 |
| F404L/N434S | (12.5) | ||
| C3A-01 | N315D/A330V/N434Y | 8.0 | MS2 |
| C3A-03 | N315D/N434Y | 6.4 | MS2 |
| C6A-05 | T307A/N315D/A330V/N434Y | 6.1 | MS2 |
| S4A-02 | A378V/N434Y | 4.6 | MS1 |
| M3A-01 | N361D/N434Y | 4.5 | MS2 |
| L6B-41 | P230S/M428L | 4.4 | MS1 |
| S4A-11 | N389T/N434Y | 3.7 | MS1 |
| S3A-35 | N434Y | 3.5 | MS1 |
| B4A-13 | P228L | 3.5 | MS1 |
| B3A-32 | P228R | 3.1 | MS1 |
| B5A-35 | P230S | 2.8 | MS1 |
| S3A-05 | N434S | 2.7 | MS1 |
| B4A-22A | A378V | 2.6 | MS1 + mutagenesis |
| B5A-25B | N315D | 2.1 | MS1 + mutagenesis |
| B5A-15 | M428L | 2.0 | MS1 |
| S3A-25A | V264E | 1.9 | MS1 + mutagenesis |
| L3A-01 | T256N | 1.8 | MS1 |
| B3A-17 | T307A | 1.3 | MS1 |
Mutations and phage-ELISA ratios obtained in comparison with the Fc-WT. Analysis of the single mutations and the different combinations of mutations allowed us to ascertain the impact of the mutations included in our six preferred variants.