Figure 2.
Elevation of nlpI in E. coli disturbed FtsZ localization. Plasmids pQE80-nlpI, pQE80-nlpI-M, pQE80-nlpI-282 and pQE80-nlpI-233 were transformed individually into E. coli strain MC1000 carrying ftsZ-yfp fusion plasmid pWM1410, the resultant strains were induced with 0.5 mM IPTG and 10 mM Ara for 4 h. Bacteria were stained with DAPI. Nucleoids and FtsZ-YFPs were observed by fluorescence microscope. Magnification, X1000. Scale bar, 10 μm.