Figure 2. Thermostabilizing mutations in PTDH.
Ribbon diagram of the thermostable PTDH variant that was used for structural studies showing the 16 thermostabilizing mutations. Two of the mutations (Glu-332→Asn and Cys-336→Asp) occur within the six carboxy-terminal residues that are not visible in the structures. Note that all of these mutations are distal to the active site.