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. 2014 Dec 18;9(3):795–800. doi: 10.3892/etm.2014.2148

Figure 1.

Figure 1

Cloning of Serratia sp. CQMUS2 ChrT gene fragments. (A) PCR amplification of the ChrT gene fragment: Lane M, DNA marker; lane 1, a 305-bp fragment obtained by PCR amplification with the Scfmn1-F and Scfmn1-R primers. (B) PCR amplification of the 5′-end DNA fragment: Lane M, DNA marker; lane 2, a 321-bp fragment obtained by the third round hiTAIL-PCR amplification with the Scfmn1-SP3 and Scfmn1-AC1 primers. (C) PCR amplification of the 3′-end DNA fragment: Lane M, DNA marker; lane 3, a 747-bp fragment obtained by the third round hiTAIL-PCR amplification with the Scfmn1-SP3′ and Scfmn1-AC1′ primers. (D) PCR amplification of the complete DNA: Lane M, DNA marker; lane 4, the 567-bp ChrT gene obtained by PCR amplification with the Scfmn-F and Scfmn-R primers. PCR, polymerase chain reaction; F, forward; R, reverse.