Table I.
Primers used in the study.
| Procedure | Primers | Primer sequences (5′-3′) |
|---|---|---|
| ChrT gene fragment | Scfmn1-F | GATGTGCAACAAGACGAAGGT |
| Scfmn1-R | GATGACTCCGCCCATAAACTC | |
| 5′-hiTAIL-PCR | Scfmn-SP1 | GATGACTCCGCCCATAAACTCC |
| Scfmn-SP2 | AGACTCACCAGAGTCGATGGCGTTTTTCAGGC | |
| Scfmn-SP3 | CTATCACAACCCCATCCGCCT | |
| Scfmn-AD1 | ACGCTAGACTCACCTCVNVNNNGGAA | |
| Scfmn-AC1 | ACGCTAGACTCACCTC | |
| 3′-hiTAIL-PCR | Scfmn-SP1′ | AGGCGGATGGGGTTGTGATAG |
| Scfmn-SP2′ | GAACTGCACACGCCTGAAAAACGCCATCGACT | |
| Scfmn-SP3′ | GTGCGCGCTGCCAGTATCAT | |
| Scfmn-AD1′ | ACGATGAACTGCACTGVVNVNNNCCAA | |
| Scfmn-AC1′ | ACGATGAACTGCACTG | |
| Full-length ChrT gene | Scfmn-F | ATCATGTCAGATACCTTGAAAGTGG |
| Scfmn-R | TGCTTTAACCCGCCGAATATA |
PCR, polymerase chain reaction; F, forward; R, reverse; hiTAIL-PCR, high efficiency TAIL-PCR.