Figure 4.
Inhibition of the growth of HepG2 cells by 6His-tagged PG-1. The cells were seeded onto 96-well plates at a density of 3×103 cells/well and treated with PG-1 at different concentrations. The percentage of cell viability was determined by an MTT assay after 1, 2, 3, 4 and 5 days of treatment. Dose- and time-dependent growth inhibition was observed at the concentrations between 20 and 160 μg/ml. The results are the mean values ± standard deviation of three independent experiments, each in six wells. PG-1, protegrin-1.