TABLE 1.
Bacterial strains used in this study
| Strain | Relevant features | Ref. |
|---|---|---|
| E. coli strains | ||
| XL1 Blue | Cloning strain, TetR--ANG127 | Stratagene |
| BL21(DE3) | E. coli strain used for protein expression--ANG191 | Novagen |
| ANG1824 | pET28b in XL1 Blue; KanR | Novagen |
| ANG1867 | pET28b in BL21 (DE3); KanR | Novagen |
| ANG2702 | pET28b-His-pstA in XL1 Blue; KanR | 9 |
| ANG2711 | pET28b-His-pstA in BL21 (DE3); KanR | 9 |
| ANG3043 | pET28b-disABT-His in XL1 Blue; KanR | This study |
| ANG3048 | pET28b-disABT-His in BL21 (DE3); KanR | This study |
| ANG3352 | pET28b-His-pstA (K2A) in XL1 Blue; KanR | This study |
| ANG3278 | pET28b-His-pstA (D12A) in XL1 Blue; KanR | This study |
| ANG3280 | pET28b-His-pstA (T28A) in XL1 Blue; KanR | This study |
| ANG3279 | pET28b-His-pstA (GGFL-AAAA) in XL1 Blue; KanR | This study |
| ANG3282 | pET28b-His-pstA (N41A) in XL1 Blue; KanR | This study |
| ANG3283 | pET28b-His-pstA (T43A) in XL1 Blue; KanR | This study |
| ANG3285 | pET28b-His-pstA (R67A) in XL1 Blue; KanR | This study |
| ANG3286 | pET28b-His-pstA (Δ71–87) in XL1 Blue; KanR | This study |
| ANG3353 | pET28b-His-pstA (K2A) in BL21 (DE3); KanR | This study |
| ANG3292 | pET28b-His-pstA (D12A) in BL21 (DE3); KanR | This study |
| ANG3293 | pET28b-His-pstA (T28A) in BL21 (DE3); KanR | This study |
| ANG3296 | pET28b-His-pstA (GGFL-AAAA) in BL21 (DE3); KanR | This study |
| ANG3294 | pET28b-His-pstA (N41A) in BL21 (DE3); KanR | This study |
| ANG3295 | pET28b-His-pstA (T43A) in BL21 (DE3); KanR | This study |
| ANG3297 | pET28b-His-pstA (R67A) in BL21 (DE3); KanR | This study |
| ANG3298 | pET28b-His-pstA (Δ71–87) in BL21 (DE3); KanR | This study |
| Other strains | ||
| BMB171 | B. thuringiensis BMB171– NG3001 | 27 |