TABLE 2.
Primers used in this study
Restriction sites are underlined.
| Number | Name | Sequence |
|---|---|---|
| ANG1591 | F-NdeI-PstA | GGGCATATGAAAATGATTATAGCGATCGTACAAG |
| ANG1592 | R-EcoRI-PstA | GGGGAATTCTTAAAATTGATGGAATGCATCAAC |
| ANG1711 | 5-NcoI-DisA-Bt | CATGCCATGGAAGAAAATAAGCAACGTGTCAAAAG |
| ANG1712 | 3-XhoI-DisA-Bt | CCGCTCGAGATTGTGTCTACTCATATAGAGATGCTCTTG |
| ANG1872 | H6.pstA (D12A)-F | GGAATTCCATATGAAAATGATTATAGCGATCGTACAAGATCAAGCTAGTCAGGAACTTGC |
| ANG1875 | H6.pstA (T28A)-R | GTTGTTGCCAATTTTGCTGCTCTAAAGTTATTTTTAAC |
| ANG1876 | H6.pstA (T28A)-F | CAGCAAAATTGGCAACAACAGGTGG |
| ANG1877 | H6.pstA (GGFL-AAAA)-R | TTGCAGCCGCAGCTGTTGTTGCCAATTTTGTTG |
| ANG1878 | H6.pstA (GGFL-AAAA)-F | CAGCTGCGGCTGCAAGAGCGGGTAATACAACATTC |
| ANG1879 | H6.pstA (N41A)-R | GCACCCGCTCTTAAAAACCCAC |
| ANG1880 | H6.pstA (N41A)-F | TTTAAGAGCGGGTGCTACAACATTCTTATGTGGTGTC |
| ANG1881 | H6.pstA (T43A)-R | TGCTGTATTACCCGCTCTTAAAAACC |
| ANG1882 | H6.pstA (T43A)-F | GCGGGTAATACAGCATTCTTATGTGGTGTCAATG |
| ANG1883 | H6.pstA (R67A)-R | TCTGCATTACCACACGTTTGATTAATC |
| ANG1884 | H6.pstA (R67A)-F | GTGTGGTAATGCAGAACAGTTGGTTTCACC |
| ANG1886 | H6.pstA (Δ71–87)-R | CAACTGTTCTCTATTACCACACGTTTG |
| ANG1887 | H6.pstA (Δ71–87)-F | AATAGAGAACAGTTGCCAGTTGAAGTTGAAGTTGG |
| ANG1931 | H6.pstA (K2A)-F | GGAATTCCATATGGCAATGATTATAGCGATCGTACAAGATCAAG |