FIGURE 2.

Comprehensive metabolic evaluations of AMI treatment. Whole-body metabolic status was assessed in AMI-treated HD mice evaluated using a CLAMS system across a variety of outputs (vehicle-treated, black line; AMI-treated, gray line), including oxygen consumption (VO₂) (A), carbon dioxide production (VCO₂) (B), RER (C), accumulated food intake (D), accumulated water intake (E), ambulatory total x axis activity (F), sleep time during the dark cycle (G), sleep time during the light cycles (H), and total sleep time during both light and dark cycles (I). Black line, vehicle-treated HD mice; gray line, AMI-treated HD mice. The same CLAMS system parameter assessment panel (J–R) was also performed in WT mice, both vehicle-treated (black line) and AMI-treated (gray line). Parameters in all panels were measured over a 24-h period. The dark cycle time is indicated by the black section of the pictogram bar in each CLAMS activity, A–F and J–O. Data are means ± S.E. *, p ≤ 0.05, n = 4/group.