FIGURE 3.

AMI-induced alterations of mHTT protein expression, neuronal marker-MAP2, and NeuN-positive cell numbers in mouse brain. A–C, immunostaining (×10×) of mutant Huntingtin protein in both vehicle-treated (Control, Con) and AMI-treated HD mice (A, cortex; B, hippocampus; C, striatum). The associated histograms for A–C indicate the reductions in mHTT immunopositive areas mediated by AMI. Data from three animals of each group are expressed as means ± S.E. *, p ≤ 0.05; **, p ≤ 0.01. D–F, Western blot verifications of mHTT changes in cortex, hippocampus, and striatum. G–I, fluorescence immunostaining of mHTT (red), MAP2 (green), and nuclei (DAPI) in the cortex (Ctx, G), hippocampus (Hip, H), and striatum (Str, I) from control (Con, vehicle-treated) or AMI-treated mice. G–I are at ×10 magnification: scale bar, 50 μm. J and K, stereological analysis of cortical and striatal NeuN-positive cells. Data from five animals of each group are expressed as means ± S.E. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.01.