FIGURE 14.
Hepatic expression of genes associated with TG secretion/hydrolysis and inflammation in HFD-treated Fsp27ΔAd (aKO) and Fsp27F/F (Flox) mice. A and B, Expression levels of genes associated with TG secretion/hydrolysis (A) and inflammation (B). C, immunoblot analysis of phosphorylated and total hormone-sensitive lipase (p-HSL and HSL, respectively) and adipose TG lipase (p-ATGL and ATGL, respectively). Liver homogenates (20 μg of protein) were loaded in each well. The band of β-actin was used as a loading control. Epididymal WAT isolated from a non-fasted and 36 h-fasted mouse was used for detecting the correct position of the bands. D, expression levels of transcription factors regulating FA/TG metabolism. Data were analyzed using two-tailed Student's t test. *, p < 0.05; **, p < 0.01 compared with Flox mice. All values represent the mean ± S.E. n = 6–8/group.