Skip to main content
. 2014 Dec 11;290(5):2831–2841. doi: 10.1074/jbc.M114.591420

FIGURE 3.

FIGURE 3.

Ectopic expression of miR-146 affects TRAF6 and IRAK1 expression. TRAF6 and IRAK1 expression in human monocytes (Mo) at day (D) 0; imDCs at days 2, 4, and 6; and mDCs at day 8 was analyzed by real-time PCR analysis (A) and Western blotting (B). There was a significant difference in TRAF6 protein (but not mRNA) expression between days 6 and 8 (p < 0.05). There was a significant difference in both IRAK1 mRNA and protein expression between days 6 and 8 (p < 0.05). imDCs at day 4 were transfected with miR-146a and/or miR-146b and scrambled control (Ctrl) inhibitors (C) or mimics (D) at a final concentration of 30 nm. Forty h later, cells were analyzed for TRAF6 and IRAK1 expression by Western blotting. Data shown are mean percentages ± S.E. and are representative of four (A and B) or three (C and D) independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.005.