TABLE 1.
Ceramide content depending on ORMDL expression levels (pmol/1 × 106cells)
Ceramide content was quantified by mass spectrometry in HEK293 cells transiently transfected with empty vector (control), ORMDL1, ORMDL2, ORMDL3, or the same total amount of the three plasmids together (ORMDL123) for 24 h and with scrambled siRNA (siControl) or siRNA for the three members of the ORMDL family (si123) for 36 h (n = 3).
| Control | ORMDL1 | ORMDL2 | ORMDL3 | ORMDL123 | siControl | si123 | |
|---|---|---|---|---|---|---|---|
| C14 | 2.1 ± 0.3 | 2.8 ± 0.1 | 3.2 ± 0.5 | 3.2 ± 0.7 | 3.4 ± 0.5 | 0.8 ± 0.1 | 1.3 ± 0.0a |
| C16 | 60.3 ± 9.0 | 58.4 ± 0.6 | 75.8 ± 14 | 75.5 ± 18 | 77.4 ± 7.6 | 14.9 ± 1.7 | 29.9 ± 2.4a |
| C16:1 | 4.8 ± 0.8 | 5.5 ± 0.3 | 6.4 ± 1.6 | 6.5 ± 1.8 | 7.4 ± 0.9 | 1.4 ± 0.1 | 2.8 ± 0.2a |
| C18 | 9.9 ± 1.9 | 6.7 ± 3.2 | 12.5 ± 2.5 | 10.4 ± 1.4 | 11.0 ± 0.3 | 2.7 ± 0.5 | 5.7 ± 0.1a |
| C20 | 3.8 ± 1.0 | 2.5 ± 0.1 | 3.9 ± 0.8 | 3.6 ± 0.2 | 3.0 ± 0.7 | 0.9 ± 0.2 | 2.3 ± 0.4b |
| C22 | 28.2 ± 4.9 | 26.0 ± 0.6 | 33.0 ± 7.4 | 27.6 ± 2.9 | 29.4 ± 0.9 | 3.7 ± 1.8 | 15.3 ± 1.5a |
| C24 | 104.9 ± 16 | 98.4 ± 1.4 | 109.5 ± 22 | 89.1 ± 7.8 | 111.1 ± 6.8 | 25.0 ± 4.1 | 47.8 ± 3.3b |
| Total | 214.1 ± 34 | 201.3 ± 3.8 | 244.3 ± 48 | 215.8 ± 33 | 242.8 ± 15 | 49.4 ± 4.8 | 105 ± 7.5a |
a p < 0.01.
b p < 0.05.