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. 2014 Dec 1;290(5):2644–2658. doi: 10.1074/jbc.M114.587667

FIGURE 6.

FIGURE 6.

Multiphase ab initio reconstruction of ETR1-ΔTM. A, global fits of the models reconstructed from data for three constructs of ETR1 (ETR1-ΔTM, ETR1DHp-CA-RD, and ETR1DHp-CA) with the model bead phases corresponding to each data set indicated. B, SDS gel of ETR1-ΔTM during different steps of the purification. Lane M, marker; lane 1, empty; lane 2, pooled fractions after the Ni-NTA step; lane 3, after the tobacco etch virus step; lane 4, after the second Ni-NTA step; lane 5, concentrated protein used for SAXS. C, rigid body refinement of ETR1-ΔTM with three homologous but dissimilar GAF domains against data of ETR1-ΔTM. D, the reconstructed multiphase models overlaid with the best rigid body model (bottom). Models on the right are rotated 90° about the vertical axis.