FIGURE 8.

Combination treatment of Ref-1 inhibitor and HMOX-1 inhibitor is synergistic under hypoxic conditions, but not low glucose. A, PaCa-2 cells were treated with increasing amounts of HMOX-1 inhibitor (Sn-PP) in combination with Ref-1 inhibitor E3330 (50 or 80 μm) simultaneously under several conditions. Top, normal oxygen; middle panel, 0.2% hypoxia; and bottom panel, low glucose. B, patient-derived cells, Pa03C were treated with increasing amounts of E3330 in combination with 25 μm Sn-PP and then placed under hypoxic conditions (0.2%). Cell survival was assessed via MTS assay 48 h after treatment for both cell lines. Error bars, ±S.E.