TABLE 1.

Primer sequences used for in vitro experiments

Gene	Sequence
β-Actin	Forward 5′-TTTTAGGATGGCAAGGGACTT-3′
	Reverse 5′-GATGAGTTGGCATGGCTTTA-3′
Occludin	Forward 5′-AAGAGTTGACAGTCCCATGGCATAC-3′
	Reverse 5′-ATCCACAGGCGAAGTTAATGGAAG-3′
ZO1	Forward 5′-TCCGTGTTGTGGATACCTTG-3′
	Reverse 5′-GGATGATGCCTCGTTCTACC-3′
ZO2	Forward 5′-AAAGCAGAGCGAACGAAGAG-3′
	Reverse 5′-TTTAGTTGCCAGACCCGTTC-3′
Claudin1	Forward 5′-CCCTATGACCCCAGTCAATG-3′
	Reverse 5′-AAGGCAGAGAGAAGCAGCAG-3′
Claudin3	Forward 5′-AAGGTGTACGACTCGCTGCT-3′
	Reverse 5′-GAAGTCCCGGATAATGGTGTT-3′
MLCK	Forward 5′-AACGAGATCAACATCATGAACCA-3′
	Reverse 5′-CAGCTGTGCTTGCTCTCGAA-3′
TNFR1	Forward 5′-CTGCCAGGAGAAACAGAACAC-3′
	Reverse 5′-AGGGATAAAAGGCAAAGACCA-3′
TNFR2	Forward 5′-TTCCAGAAAACCCCAGCA-3′
	Reverse 5′-TGGCCTGAGGTGATGCTT-3′
GPR40	Forward 5′-GCCCACTTCTTCCCACTCT-3′
	Reverse 5′-ACCAGACCCAGGTGACACA-3′
GPR120	Forward 5′-TCTCGTGGGATGTCTCTTTTG-3′
	Reverse 5′-CTTACCGTGAGCCTCTTCCTT-3′
PPARγ	Forward 5′-ATGGAGCCCAAGTTTGAGTTT-3′
	Reverse 5′-TGTCTGAGGTCCGTCATTTTC-3′
GPR41	Forward 5′-CTTCATCCTCTGCCCACTCTC-3′
	Reverse 5′-CGCAGATATAGCCCACGACAT-3′
GPR43	Forward 5′-ACGCAGAGGCAAAGACACA-3′
	Reverse 5′-TCTGTCGCTAGGCTGGAGT-3′