TABLE 2.

Primer sequences used for in vivo experiments

Gene	Sequence
GAPDH	Forward 5′-TCAAGAAGGTGGTGAAGCAG-3′
	Reverse 5′-AAGGTGGAAGAGTGGGAGTTG-3′
Occludin	Forward 5′-CACACTTGCTTGGGACAGAG-3′
	Reverse 5′-TAGCCATAGCCTCCATAGCC-3′
ZO1	Forward 5′-CTTCTCTTGCTGGCCCTAAAC-3′
	Reverse 5′-TGGCTTCACTTGAGGTTTCTG-3′
ZO2	Forward 5′-AACGGATGCTGGAAGTTAAT-3′
	Reverse 5′-TCTGCTTGCTGTCTCTCAACA-3′
Claudin1	Forward 5′-GATGTGGATGGCTGTCATTG-3′
	Reverse 5′-CCTGGCCAAATTCATACCTG-3′
Claudin3	Forward 5′-AACTGCGTACAAGACGAGACG-3′
	Reverse 5′-ATCCCTGATGATGGTGTTGG-3′
MLCK	Forward 5′-GCGTGATCAGCCTGTTCTTTCTAA-3′
	Reverse 5′-GCCCCATCTGCCCTTCTTTGACC-3′
TNFR1	Forward 5′-AAAGGTCTCTAAGGGGGAAGG-3′
	Reverse 5′-CAAGTGGGCACCAGATACATT-3′
TNFR2	Forward 5′-GCCATCCCAAGGACACTCTA-3′
	Reverse 5′-AGGGCTTCTTTTTCCTCTGC-3′