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. 2014 Dec 10;290(5):2919–2937. doi: 10.1074/jbc.M114.611251

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — ExoU_{P. aer}, ExoU_{P. flu}, and ExoU_{P. asy} localize to the plasma membrane of yeast in a PI(4,5)P₂-dependent fashion. A, PI(4,5)P₂ localization was visualized in wild-type (WT) and Δinp54/Δsac1 yeast by the expression of GFP-tagged pleckstrin homology domain from phospholipase C. Yeast were visualized using fluorescence confocal microscopy. B, GFP-tagged catalytically inactive ExoU proteins were visualized by fluorescence confocal microscopy following expression in yeast. The insets show individual yeast cells magnified ×15. ExoU_{P. asy}-MLD refers to the membrane localization domain of ExoU_{P. asy} Scale bars, 25 μm.